Környezetbarát eljárás kifejlesztése arany nanorészecskék előállítására és azok alkalmazása bioszenzor érzékenyítésére
Development of an environment-friendly process for the production of gold nanoparticles and their use for biosensor signal amplification
Project ID: HU09-0118-A2-2016
|Projektgazda/ Project Promoter||Fermentia Mikrobiológiai Kft.|
Program Operátor/Programme Operator
Szent István Egyetem Szent István University
Megvalósítási időszak/Implementation period
2016.05.01. - 2017.04.30 / 01.05.2016. -30.04.2017.
Hazai Projekt Partnerek/National Project Partner
NAIK / National Agricultural Research and Innovation Centre
BME, Természettudományi kar / BME Faculty of Natural Sciences
Donor Projekt Partner/Donor Project Partner
HSN College of Southeast NorwayDepartment of Micro- and Nanosystem Technology
Összköltség / Total budget(EUR)
81 254 829 HUF/ 267 549,65 EUR
EGT/Norvég támogatás összege / EEA/Norwegian Grant (EUR)
72 572 696 HUF/ 238 961,79 EUR
There are several chemical and physical methods to produce AuNPs, but these have a high energy demand and can be harmful on the environment. On the other side, production of BioAuNPs by microorganisms can be considered a „green” process.
During our project we have developed a process for synthesising of AuNPs by thermophilic fungi, and the following purification steps.
The produced colloid solutions have been characterized by several analytical methods (UV-VIS spectrophotometry, dynamic light scattering (DLS), transmission electron microscopy (TEM)).
The efficiency of the bioAuNPs was verified by the developed measurement method for the BSA – anti-BSA model molecule pair. Then with the application of bioAuNPs a highly sensitive, selective, rapid measurement procedure was elaborated on the detection of aflatoxin B1 mycotoxin from food samples.
The process for QCM-I (Quartz Crystal Microbalance) was capable of detecting aflatoxin B1 from spiced paprika matrix in the concentration range of 2-100 ng/ml (IC50=65,6±0,005 ng/mL), with a detection limit of 1 ng/ml. The developed immunosensor for OWLS (Optical Waveguide Lightmode Spectroscopy) system functioned successfully in the concentration range of 0,001-1,000 ng/mL (IC50 0,035±0,005 ng/mL), whereas the sensitivity without AuNP was only 1,000-20,000 ng/mL, which is a remarkable improvement.
Based on our results it can be confirmed, that the application of bioAuNPs is a potential alternative to chemically synthetised nanoparticles. Furthermore, sensitization of QCM-I and OWLS immunosensors with BioAuNPs resulted in a rapid and cheap method for the detection of aflatoxin B1 mycotoxin, more sensitive than other previously used methods.